SECRETION OF IMMUNOREACTIVE ENDOTHELIN-1 BY CAPILLARY AND MICROVASCULAR ENDOTHELIUM OF HUMAN BRAIN

被引：64

作者：

BACIC, F

UEMATSU, S

MCCARRON, RM

SPATZ, M

机构：

[1] NINCDS, STROKE BRANCH, BLDG 36, ROOM 4D04, BETHESDA, MD 20892 USA

[2] JOHNS HOPKINS UNIV HOSP, BALTIMORE, MD 21205 USA

来源：

NEUROCHEMICAL RESEARCH | 1992年 / 17卷 / 07期

关键词：

ENDOTHELIN-1; CAPILLARIES; VASOCONSTRICTOR PEPTIDE;

D O I：

10.1007/BF00968008

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Modulation of immunoreactive endothelin-1 (IR-ET-1) production by vasoactive substances was investigated in cultured endothelial cells (EC) derived from capillaries and microvessels of human brain. Peptides, catecholamines, thrombin, protein kinase C-activating phorbol ester, and calcium ionophore enhanced the secretion of IR-ET-1. The known vasoconstrictive peptides, angiotensin II (Ang II) and arginine-vasopressin (AVP) dose-dependently stimulated the endothelial secretion of IR-ET-1. The angiotensin and vasopressin-inducible production of IR-ET-1 was completely inhibited by their respective receptor antagonists [Sar1, Ala8]-angiotensin II and [1-6 (beta-mercapto-beta,beta-cyclopentamethylene propionic acid), 2-O-methyl-tyrosine]. The results indicate that the peptide-stimulated secretion of IR-ET-1 is receptor-mediated in EC which have specific angiotensin II and arginine-vasopressin receptors. These findings represent the first demonstration of IR-ET-1 production by capillary and microvascular endothelium of human brain.

引用

页码：699 / 702

页数：4

共 17 条

[1] HUMAN CULTURED ENDOTHELIAL-CELLS DO SECRETE ENDOTHELIN-1 [J].