COMBINED PRODUCT AND SUBSTRATE-INHIBITION EQUATION FOR CELLOBIASE

Cellobiase (CE 3.2.1.21) is a β-glucosidase which hydrolyzes cellobiose to glucose and is known to be subject to both product and substrate inhibition. This work report a model which combines both product and substrate inhibition effects for cellobiase isolated from a commercial preparation of Trichoderma viride from Miles Laboratories (Elkhart, IN). An integrated rate equation is presented which predicts the trends of time courses for hydrolyses of cellobiose a t concentrations ranging from 14.6-1416mM cellobiose. The constants used in the model (determined from initial rate data) are compared to those reported for cellobiase obtained from other sources of T. Viride. Most notable in this comparison is the apparently higher activity and reduced inhibition of this enzyme compared to other sources of cellobiase. © 1981 John Wiley & Sons, Inc.