TNF-ALPHA MESSENGER-RNA EXPRESSION IN DIAPHRAGM MUSCLE AFTER ENDOTOXIN ADMINISTRATION

被引：61

作者：

SHINDOH, C ^{[1
]}

HIDA, W ^{[1
]}

OHKAWARA, Y ^{[1
]}

YAMAUCHI, K ^{[1
]}

OHNO, I ^{[1
]}

TAKISHIMA, T ^{[1
]}

SHIRATO, K ^{[1
]}

机构：

[1] TOHOKU UNIV,SCH MED,DEPT INTERNAL MED 1,AOBA KU,SENDAI,MIYAGI 980,JAPAN

来源：

AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE | 1995年 / 152卷 / 05期

关键词：

D O I：

10.1164/ajrccm.152.5.7582314

中图分类号：

R4 [临床医学];

学科分类号：

1002 ; 100602 ;

摘要：

We studied gene expression and production of TNF-alpha in the diaphragm tissue and changes of muscle contractile properties after endotoxin injection (Escherichia coli, 20 mg/kg) in 88 rats. We assessed the muscle contractile properties by force-frequency curves and twitch kinetics using dissected diaphragm muscle strips. The peak tensions of force-frequency curves decreased from control values (2.15 +/- 0.2 kg/cm(2)) up to 4 h (0.81 +/- 0.17, p < 0.001), and then increased at 6 h (1.36 +/- 0.19, p < 0.05) after endotoxin injection. The cytotoxic activity on L929 cells in arterial blood samples maximally increased at 2 h (p < 0.001), then decreased to 6 h (p < 0.05). TNF-alpha mRNA in diaphragm tissue was detected by Northern blot method at 1 and 1.5 h, and the immunolocalization of TNF-alpha was evaluated at 2 and 4 h by immunohistochemistry in the muscle tissues. Furthermore, preinjection with anti-m TNf-alpha antibody prevented the decrement of force-frequency curves after endotoxin injection of 10 mu l/kg. From this evidence that TNF-alpha gene expression and production occurred in the diaphragm tissue, but anti-m TNF-alpha antibody preinjection prevented the deterioration of diaphragm muscle contractile properties, we suggest that TNF-alpha may act on muscle cells extracellularly.

引用

页码：1690 / 1696

页数：7

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