TETRATHIAFULVALENE TETRACYANOQUINODIMETHANE XANTHINE-OXIDASE AMPEROMETRIC ELECTRODE FOR THE DETERMINATION OF BIOLOGICAL PURINES

被引：82

作者：

MCKENNA, K ^{[1
]}

BRAJTERTOTH, A ^{[1
]}

机构：

[1] UNIV FLORIDA,DEPT CHEM,GAINESVILLE,FL 32611

来源：

ANALYTICAL CHEMISTRY | 1987年 / 59卷 / 07期

关键词：

BIOLOGICAL MATERIALS - Blood - ENZYMES - Applications;

D O I：

10.1021/ac00134a006

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An amperometric xanthine oxidase electrode constructed with the conducting salt tetrathiafulvalene tetracyanoquinodimethane, TTF** plus TCNQ** minus , as the electrode material is evaluated for the determination of purine, hypoxanthine, and xanthine. Under unsaturated enzyme conditions, substrate-dependent current response is linear in the concentration range 10** minus **5 to 6 multiplied by 10** minus **4 M. Substrate diffusion through the membrane is the rate-limiting step. Application to the determination of purines in blood plasma is described.

引用

页码：954 / 958

页数：5

共 20 条

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