SIMPLE PROCEDURE FOR OBTAINING AUTORADIOGRAPHS OF G-BANDED CHROMOSOMES

被引：7

作者：

RUTLEDGE, MH

机构：

[1] Department of Radiology and Radiation Biology, Colorado State University, Fort Collins, 80523, Colorado

来源：

CHROMOSOMA | 1979年 / 70卷 / 02期

关键词：

D O I：

10.1007/BF00288410

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Chromosomal preparations from cells flash-labeled with 3H-dT were Giemsa-banded following trypsin digestion, allowed to air-dry, and then were coated with a layer of 1% Formvar. The slides were subsequently coated with radiographic emulsion (NTB2) and processed for autoradiography. The resulting chromosomes had distinct G-banding and radiographic labeling patterns. Chemographic grain formation in the emulsion, normally caused by Giemsa stain, was prevented by the film of Formvar, allowing a very low background grain level. © 1979 Springer-Verlag.

引用

页码：259 / 262

页数：4