TRIPLOIDY AFTER INVITRO FERTILIZATION - CYTOGENETIC ANALYSIS OF HUMAN ZYGOTES AND EMBRYOS

被引：50

作者：

PIETERS, MHEC

DUMOULIN, JCM

IGNOULVANVUCHELEN, RCM

BRAS, M

EVERS, JLH

GERAEDTS, JPM

机构：

[1] Department of Genetics/Cell Biology, University of Limburg, Maastricht, 6200 MD

[2] Department of Obstetrics/Gynaecology, University of Limburg, Maastricht, 6202 AZ MD

来源：

JOURNAL OF ASSISTED REPRODUCTION AND GENETICS | 1992年 / 9卷 / 01期

关键词：

INVITRO FERTILIZATION (IVF); ZYGOTES; EMBRYOS; TRIPLOIDY; CHROMOSOMAL MOSAICISM;

D O I：

10.1007/BF01204118

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Tripronuclear zygotes obtained from a clinical IVF program were studied cytogenetically. Successful analysis was possible of 42 specimens at the zygote stage and 21 embryos after the first or second cleavage division. In the majority of zygotes (88%) the expected triploidy was confirmed, whereas only 14% of embryos had solely triploid cells. Therefore it is concluded that after tripolar cleavage division, many different types of mosaicism may originate from irregular chromosome distributions. Since the findings in individual blastomeres in embryos resulting from multipronuclear zygotes do not reflect the genetic content of the whole embryo, these embryos are less suitable in a model system for preimplantion diagnosis. The distribution of the sex chromosomal types (XXX, XXY, and XYY) confirmed theoretical expectations. Since in abortion material or in liveborn triploidy cases, the XYY karyotype is hardly ever observed, this indicates that most likely the 69,XYY karyotype has a very high embryonic mortality.

引用

页码：68 / 76

页数：9

共 26 条

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