A SINGLE-REACTION METHOD FOR DNA-SEQUENCE DETERMINATION

被引：26

作者：

NEGRI, R ^{[1
]}

COSTANZO, G ^{[1
]}

DIMAURO, E ^{[1
]}

机构：

[1] UNIV ROME LA SAPIENZA,DIPARTIMENTO GENET & BIOL MOLEC,I-00185 ROME,ITALY

来源：

ANALYTICAL BIOCHEMISTRY | 1991年 / 197卷 / 02期

关键词：

D O I：

10.1016/0003-2697(91)90409-M

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A chemical method for the determination of DNA sequence is presented. Heating of DNA, labeled at a single 3′ extremity, in the presence of formamide results in efficient cleavage of phosphodiester bonds 3′ of A, G, and C residues. The relative efficiency is A = G > C. The bias between A and G is solved by a simple pretreatment photoreaction in methylene blue) followed by heating in formamide. The entire procedure does not require any intermediate purification step or handling of hazardous chemicals and allows determination of the sequence on only two electrophoretic lanes. Its simplicity and rapidity favor automatization. © 1991.

引用

页码：389 / 395

页数：7

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