CRYSTALLIZATION STUDIES ON HIV-1 REVERSE-TRANSCRIPTASE

被引：2

作者：

LLOYD, LF ^{[1
]}

BRICK, P ^{[1
]}

BLOW, DM ^{[1
]}

LOU, MZ ^{[1
]}

机构：

[1] ACAD SINICA,INST BIOPHYS,BEIJING 100080,PEOPLES R CHINA

来源：

JOURNAL OF CRYSTAL GROWTH | 1992年 / 122卷 / 1-4期

关键词：

D O I：

10.1016/0022-0248(92)90269-O

中图分类号：

O7 [晶体学];

学科分类号：

0702 ; 070205 ; 0703 ; 080501 ;

摘要：

HIV-1 reverse transcriptase has been crystallized in a variety of forms. Various ligands used for co-crystallization are described and the results presented. All of these crystals showed disorder when examined in the X-ray beam. The best diffraction currently achieved has been approximately 7 angstrom. The possible reasons for crystal disorder are discussed. An example of another protein, carboxypeptidase G2, Which initially yielded non-diffracting crystals, is used to illustrate the value of applying random or incomplete factorial screens to sample wider parameter space for conditions to grow well-ordered crystals.

引用

页码：355 / 359

页数：5

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