PRODUCTION OF SPECIFIC ANTIBODY AGAINST L-DOPA AND ITS ULTRASTRUCTURAL-LOCALIZATION OF IMMUNOREACTIVITY IN THE HOUSE-SHREW (SUNCUS-MURINUS) LATERAL HABENULAR NUCLEUS

被引：30

作者：

KARASAWA, N

ISOMURA, G

NAGATSU, I

机构：

[1] Department of Anatomy, School of Medicine, Fujita Health University, Aichi

来源：

NEUROSCIENCE LETTERS | 1992年 / 143卷 / 1-2期

关键词：

L-3,4-DIHYDROXYPHENYLALANINE; TYROSINE HYDROXYLASE; AROMATIC L-AMINO ACID DECARBOXYLASE; DOPAMINE; CATECHOLAMINE; IMMUNOHISTOCHEMISTRY; LATERAL HABENULAR NUCLEUS; HOUSE-SHREW;

D O I：

10.1016/0304-3940(92)90280-K

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

An antiserum was raised against L-DOPA bound to bovine serum albumin, purified by affinity chromatography, and its specificities were verified by immunoblotting and enzyme-linked immunosorbent assays. The antiserum did not cross-react with dopamine (DA), tyrosine, tyramine, octopamine, norepinephrine or epinephrine. Immunocytochemical studies using the PAP method revealed that tyrosine hydroxylase- and L-DOPA positive but aromatic L-amino acid decarboxylase- and DA-negative neurons were present in the lateral habenular nucleus of the house-shrew (Suncus murinus) brain. Ultrastructurally L-DOPA immunoreactive products were localized in the cytoplasmic matrix and terminals with vesicles.

引用

页码：267 / 270

页数：4

共 11 条

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