PURIFICATION AND CHARACTERIZATION OF AN ACETYL ESTERASE FROM ASPERGILLUS-NIGER

被引：30

作者：

LINDEN, J

SAMARA, M

DECKER, S

JOHNSON, E

BOYER, M

PECS, M

ADNEY, W

HIMMEL, M

机构：

[1] TECH UNIV BUDAPEST, INST AGR CHEM TECHNOL, BUDAPEST, HUNGARY

[2] NATL RENEWABLE ENERGY LAB, DIV ALTERNAT FUELS, GOLDEN, CO USA

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 1994年 / 45-6卷

关键词：

ACETYL ESTERASE; ACETYL XYLAN ESTERASE; ASPERGILLUS NIGER; HEMICELLULOSE;

D O I：

10.1007/BF02941813

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Optimized acetyl esterase enzyme production conditions using Aspergillus niger ATCC 10864 in 14-L fermentation jars were determined to be 33 degrees C, 1.5 vvm aeration, and 300 rpm agitation without pH control. The acetyl esterase was purified by precipitation in 60-80% saturation in ammonium sulfate. The pellet was applied directly to a Pharmacia high-load Phenyl Sepharose column for hydrophobic interaction chromatography and purified to homogeneity in two steps.

引用

页码：383 / 393

页数：11

共 14 条

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