STIMULATION OF CULTURED AMNION CELL PROSTAGLANDIN ENDOPEROXIDE H-SYNTHASE ACTIVITY BY GLUCOCORTICOIDS AND PHORBOL ESTER

被引:17
作者
SMIEJA, Z
ZAKAR, T
OLSON, DM
机构
[1] UNIV ALBERTA,DEPT OBSTET & GYNAECOL,PERINATAL RES CTR,660 HERITAGE MED RES CTR,EDMONTON T6G 2S2,AB,CANADA
[2] UNIV WESTERN ONTARIO,ST JOSEPHS HLTH CTR,LAWSON RES INST,DEPT PHYSIOL,MRC,LONDON N6A 3K7,ONTARIO,CANADA
[3] UNIV WESTERN ONTARIO,ST JOSEPHS HLTH CTR,LAWSON RES INST,DEPT PAEDIAT,LONDON N6A 3K7,ONTARIO,CANADA
[4] UNIV ALBERTA,DEPT PEDIAT,EDMONTON T6G 2E1,ALBERTA,CANADA
[5] UNIV ALBERTA,DEPT PHYSIOL,EDMONTON T6G 2E1,ALBERTA,CANADA
关键词
PROSTAGLANDIN H-SYNTHASE OR PROSTAGLANDIN ENDOPEROXIDE SYNTHASE; CYCLOOXYGENASE; PROSTAGLANDINS; AMNION;
D O I
10.1016/0002-9378(93)90639-Z
中图分类号
R71 [妇产科学];
学科分类号
100211 ;
摘要
OBJECTIVE: The effects of a phorbol ester activator of protein kinase C and the glucocorticoids cortisol and dexamethasone on the enzyme activity of prostaglandin H synthase (cyclooxygenase) in confluent cultures of human amnion epithelial cells were tested. STUDY DESIGN: Amnion epithelial cells from spontaneously delivered placentas at term were isolated and grown in culture until confluent. The activity of the enzyme prostaglandin H synthase was determined in these cells with a well-characterized enzyme assay monitoring the conversion of arachidonic acid to prostaglandin E2. The Michaelis-Menten constant and maximum velocity were determined from the substrate velocity data by means of Lineweaver-Burk plots. RESULTS: Amnion cells lost most of their prostaglandin H synthase activity within 2 days of culturing. This activity could be restored when cells were treated with 12-O-tetradecanoyl phorbol-13-acetate, a phorbol ester activator of protein kinase C, or with the glucocorticoids cortisol and dexamethasone. Epidermal growth factor also increased the specific activity, whereas 17beta-estradiol had no effect on the specific activity of the enzyme. There was a direct correlation between the specific activity of prostaglandin H synthase and the output of prostaglandin E2 by cells treated with these agonists. CONCLUSION: Our data support the view that increases in prostaglandin H synthase specific activity in intrauterine tissues can be caused by stimulation by specific agonists, and this in turn is responsible for enhanced prostaglandin output by these tissues.
引用
收藏
页码:653 / 661
页数:9
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