DISTRIBUTION OF GABA(A) AND GABA(B) BINDING-SITES IN THE COCHLEAR NUCLEUS OF THE GUINEA-PIG

被引:34
作者
JUIZ, JM
ALBIN, RL
HELFERT, RH
ALTSCHULER, RA
机构
[1] UNIV MICHIGAN, KRESGE HEARING RES INST, ANN ARBOR, MI 48109 USA
[2] UNIV MICHIGAN, DEPT NEUROL, ANN ARBOR, MI 48104 USA
[3] UNIV MICHIGAN, NEUROSCI PROGRAM, ANN ARBOR, MI 48104 USA
关键词
GABA; GABA RECEPTOR; AUDITORY PATHWAY; COCHLEAR NUCLEUS; NEUROTRANSMITTER;
D O I
10.1016/0006-8993(94)91730-2
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
We compared the distribution of GABA(A) and GABA(B) binding sites in the cochlear nucleus using quantitative receptor autoradiography with [H-3]GABA. To visualize GABA(A) binding sites, GABA(B) binding sites were blocked with +/-baclofen. To visualize GABA(B) binding sites, isoguvacine was used to block GABA(A) binding sites. GABA(A) binding sites predominated over GABA(B), although there were marked regional differences in the distribution of binding. In the ventral cochlear nucleus, GABAA and GABA(B) binding sites were concentrated in the peripheral granule cell cap, with low binding levels in the central region. In the dorsal cochlear nucleus, binding was concentrated in the superficial (fusiform and molecular) layers, with a distinct laminar pattern. GABA(A) binding sites predominated in the fusiform cell layer. The molecular layer contained the highest level of GABA(B) binding sites in the entire cochlear nucleus. These results suggest that GABAergic inhibition in the cochlear nucleus is mediated both by GABA(A) and GABA(B) receptors, particularly in the dorsal cochlear nucleus. However, low levels of binding in areas such as the magnocellular regions of the ventral cochlear nucleus, known to contain abundant GABAergic synapses, suggest heterogeneity of GABA receptors in this auditory nucleus.
引用
收藏
页码:193 / 201
页数:9
相关论文
共 85 条
[1]  
ALFORD S, 1991, J NEUROSCI, V11, P3718
[2]   NEUROTRANSMITTERS OF THE COCHLEA AND COCHLEAR NUCLEUS - IMMUNOCYTOCHEMICAL EVIDENCE [J].
ALTSCHULER, RA ;
HOFFMAN, DW ;
WENTHOLD, RJ .
AMERICAN JOURNAL OF OTOLARYNGOLOGY, 1986, 7 (02) :100-106
[3]  
ALTSCHULER RA, 1993, NATO ADV SCI INST SE, V239, P211
[4]   EFFECTS OF THE MURINE MUTATION NERVOUS ON NEURONS IN CEREBELLUM AND DORSAL COCHLEAR NUCLEUS [J].
BERREBI, AS ;
MUGNAINI, E .
JOURNAL OF NEUROCYTOLOGY, 1988, 17 (04) :465-484
[5]   PYRAMIDAL NEURONS OF THE DORSAL COCHLEAR NUCLEUS - A GOLGI AND COMPUTER RECONSTRUCTION STUDY IN CAT [J].
BLACKSTAD, TW ;
OSEN, KK ;
MUGNAINI, E .
NEUROSCIENCE, 1984, 13 (03) :827-854
[6]   GABAB RECEPTORS AND THEIR SIGNIFICANCE IN MAMMALIAN PHARMACOLOGY [J].
BOWERY, N .
TRENDS IN PHARMACOLOGICAL SCIENCES, 1989, 10 (10) :401-407
[7]  
Bowery N. G, 1984, GABA RECEPTORS, P177
[8]   GABA-A AND GABA-B RECEPTOR-SITE DISTRIBUTION IN THE RAT CENTRAL-NERVOUS-SYSTEM [J].
BOWERY, NG ;
HUDSON, AL ;
PRICE, GW .
NEUROSCIENCE, 1987, 20 (02) :365-383
[9]   GABA RECEPTOR MULTIPLICITY - VISUALIZATION OF DIFFERENT RECEPTOR TYPES IN THE MAMMALIAN CNS [J].
BOWERY, NG ;
PRICE, GW ;
HUDSON, AL ;
HILL, DR ;
WILKIN, GP ;
TURNBULL, MJ .
NEUROPHARMACOLOGY, 1984, 23 (2B) :219-231
[10]   (-) BACLOFEN DECREASES NEUROTRANSMITTER RELEASE IN THE MAMMALIAN CNS BY AN ACTION AT A NOVEL GABA RECEPTOR [J].
BOWERY, NG ;
HILL, DR ;
HUDSON, AL ;
DOBLE, A ;
MIDDLEMISS, DN ;
SHAW, J ;
TURNBULL, M .
NATURE, 1980, 283 (5742) :92-94