BIOSYNTHESIS AND PHOSPHATE CONTROL OF CANDICIDIN BY STREPTOMYCES-ACRIMYCINI JI2236 - EFFECT OF AMPLIFICATION OF THE PABAB GENE

被引:15
作者
ASTURIAS, JA [1 ]
MARTIN, JF [1 ]
LIRAS, P [1 ]
机构
[1] UNIV LEON,FAC BIOL,AREA MICROBIOL,E-24071 LEON,SPAIN
来源
JOURNAL OF INDUSTRIAL MICROBIOLOGY | 1994年 / 13卷 / 03期
关键词
PHOSPHATE REGULATION; CANDICIDIN; STREPTOMYCES-ACRIMYCINI; PABA SYNTHASE; PABAB;
D O I
10.1007/BF01584005
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Biosynthesis of candicidin by Streptomyces acrimycini JI2236 was strongly inhibited by phosphate. p-Aminobenzoic acid (PABA) synthase activity, required for the synthesis of PABA, a candicidin precursor, was reduced by 72% in cells grown in medium supplemented with 7.5 mM phosphate. Hybridization studies showed that the DNA region of S. acrimycini carrying the pabAB gene (encoding PABA synthase) is very similar to the homologous region of S. griseus 3570. S. acrimycini was easily transformed with plasmids containing the pabAB gene of S. griseus. Four transformants were studied in detail; three of the transformants synthesized higher levels of PABA synthase and two transformants produced more candicidin than control cultures transformed with pIJ699. The fourth transformant was unable to synthesize the antibiotic. Formation of PABA synthase and candicidin production was equally sensitive to phosphate regulation in transformants with the pabAB than in the untransformed S. acrimycini strain.
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页码:183 / 189
页数:7
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