FETAL BOVINE SERUM INHIBITS CHONDROCYTE COLLAGENASE PRODUCTION - INTERLEUKIN-1 REVERSES THIS EFFECT

被引：16

作者：

KANDEL, RA

PRITZKER, KPH

MILLS, GB

CRUZ, TF

机构：

[1] MT SINAI HOSP,DEPT CLIN BIOCHEM,TORONTO M5G 1X5,ONTARIO,CANADA

[2] UNIV TORONTO,TORONTO GEN HOSP,DEPT IMMUNOL,TORONTO M5G 1L7,ONTARIO,CANADA

[3] UNIV TORONTO,TORONTO GEN HOSP,DEPT ONCOL RES,TORONTO M5G 1L7,ONTARIO,CANADA

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1990年 / 1053卷 / 2-3期

关键词：

Chondrocyte; Collagenase; Fetal bovine serum; Interleukin; 1;

D O I：

10.1016/0167-4889(90)90004-W

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Bovine articular chondrocytes incubated in medium which was serum free or contained low levels of fetal bovine serum (less than 5%) constitutively produced collagenase. Increasing the concentration of serum in the culture medium inhibited the production of collagenase. Addition of interleukin 1 and lipopolysaccharide reversed the inhibitory effect of serum. Phorbol esters only stimulated collagenase production when the serum concentration was at least 10%. These data suggest that there is a factor(s) in fetal bovine serum that inhibits collagenase production by chondrocytes and this can be reversed by agents such as interleukin 1. © 1990.

引用

页码：130 / 134

页数：5

共 25 条

[1] GLUCOCORTICOID MODULATION OF COLLAGENASE EXPRESSION IN HUMAN-SKIN FIBROBLAST-CULTURES - EVIDENCE FOR PRE-TRANSLATIONAL INHIBITION [J].