A MICROTITER PLATE-BASED ASSAY FOR PHOSPHOENOLPYRUVATE CARBOXYLASE

被引：4

作者：

COCKBURN, W ^{[1
]}

WHITELAM, GC ^{[1
]}

SLOCOMBE, SP ^{[1
]}

MCKEE, RA ^{[1
]}

机构：

[1] INST FOOD RES, NORWICH NR4 7UA, NORFOLK, ENGLAND

来源：

ANALYTICAL BIOCHEMISTRY | 1990年 / 189卷 / 01期

关键词：

D O I：

10.1016/0003-2697(90)90051-A

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A sensitive, quantitative assay for phosphenolpyruvate carboxylase which utilizes microtiter plates is described. The assay depends upon the production of a colored compound in the reaction between oxaloacetate, the product of the phosphoenolpyruvate reaction, and the dye Fast Violet B. The method is particularly appropriate for monitoring chromatographic eluates and its utility for this purpose is demonstrated by the detection of phosphoenolpyruvate carboxylase in fractions of crude maize extract separated by size-exclusion chromatography. © 1990.

引用

页码：95 / 98

页数：4

共 7 条

[1]

BANDURSKI RS, 1953, J BIOL CHEM, V204, P781

[2] SALT STRESS LEADS TO DIFFERENTIAL EXPRESSION OF 2 ISOGENES OF PHOSPHOENOLPYRUVATE CARBOXYLASE DURING CRASSULACEAN ACID METABOLISM INDUCTION IN THE COMMON ICE PLANT [J].