GROWTH AND COMPOSITION OF ESCHERICHIA-COLI SUBJECTED TO SQUARE-WAVE PERTURBATIONS IN NUTRIENT SUPPLY - EFFECT OF VARYING FREQUENCIES

被引：34

作者：

PICKETT, AM ^{[1
]}

BAZIN, MJ ^{[1
]}

TOPIWALA, HH ^{[1
]}

机构：

[1] SHELL RES LTD, SITTINGBOURNE RES CTR, SITTINGBOURNE ME9 8AG, KENT, ENGLAND

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 1979年 / 21卷 / 06期

关键词：

D O I：

10.1002/bit.260210609

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The effect of regularly varying the input nutrient concentration on the growth and composition (elemental and macromolecular) of E. coli ML30 grown in chemostat culture is reported. After each change in substrate concentration a lag between the stimulus and organism response occurred, the magnitude of which was dependent upon the frequency of the perturbations. Both the growth yield and macromolecular composition of the cells varied with the frequency of nutrient cycling: the yield decreased by 10% as the cycle-times decreased from 6.0 to 0.25 h. Maintaining microoorganisms under controlled transient conditions might be useful in increasing yields of specific microbial products (e.g., single-cell protein).

引用

页码：1043 / 1055

页数：13

共 20 条

[1] UNUSED PROTEIN SYNTHETIC CAPACITY OF ESCHERICHIA-COLI GROWN IN PHOSPHATE-LIMITED CHEMOSTATS [J].

ALTON, TH ;

KOCH, AL .

JOURNAL OF MOLECULAR BIOLOGY, 1974, 86 (01) :1-9

[2]

BAZIN MJ, 1975, 10TH P INT COULT EL, P426

[3] RESPONSE OF A CONTINUOUS ANAEROBIC CULTURE TO PERIODIC VARIATION OF FEEDING MASH CONCENTRATION [J].

BORZANI, W ;

GREGORI, RE ;

VAIRO, MLR .

BIOTECHNOLOGY AND BIOENGINEERING, 1976, 18 (05) :623-632

[4]

Bryant J., 1977, ADV BIOCHEM ENG, V5, P101

[5] STUDY OF THE CONDITIONS AND MECHANISM OF THE DIPHENYLAMINE REACTION FOR THE COLORIMETRIC ESTIMATION OF DEOXYRIBONUCLEIC ACID [J].

BURTON, K .

BIOCHEMICAL JOURNAL, 1956, 62 (02) :315-323

[6] KINETICS OF RIBOSOME SYNTHESIS DURING A NUTRITIONAL SHIFT-UP IN ESCHERICHIA-COLI K-12 [J].

CHAMPNEY, WS .

MOLECULAR & GENERAL GENETICS, 1977, 152 (03) :259-266

[7]

DISCHE Z, 1955, NUCLEIC ACIDS, V1, P680

[8]

ELSWORTH R., 1956, JOUR APPL BACT, V19, P264, DOI 10.1111/j.1365-2672.1956.tb00077.x

[9]

GOW JS, 1975, SINGLE CELL PROTEIN, V2, P370

[10]

Harrison DEF., 1974, ADV BIOCHEM ENG, V3, P167

← 1 2 →