COATED-OPEN-TUBULAR CHROMATOGRAPHY WITH FLOW SEGMENTATION .2. EXPERIMENTAL-STUDY AND OPTIMIZATION FOR SIZE-EXCLUSION SEPARATION

Preliminary experimental results are reported on the efficiency of segmented- flow liquid chromatography (SF-LC) as a function of experimental conditions. For agarose-coated tubes (sizes-exclusion mode) experimenta H values are significantly larger than predicted by theory. This has been traced to a combination of hydraulic istability (unless special precautions are taken) and uneven coating of the stationary phase onto the walls of the sepration tube. Further work should bead to a close agreement between theoretical and experimental separation efficiencies. The use of SL-LC as a technique for sample pretreatment prior to high- performance liquid chromatography is discribed. Serum samples for analysis of certain therapeutic drugs are subjected to deproteinization and drug release by protein precipitation, followed by SF-LC separation of released drugs from the particulate material comprising the protein. © 1979.