MOLECULAR-CLONING OF CDNA-ENCODING THE 16KDA SUBUNIT OF VACUOLAR H+-ATPASE FROM MOUSE CEREBELLUM

被引：53

作者：

HANADA, H

HASEBE, M

MORIYAMA, Y

MAEDA, M

FUTAI, M

机构：

[1] Department of Organic Chemistry and Biochemistry, Institute of Scientific and Industrial Research, Osaka University, Ibaraki, Osaka, 567

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1991年 / 176卷 / 03期

关键词：

D O I：

10.1016/0006-291X(91)90391-J

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

cDNa for the 16 kDa subunit of vacuolar H+-ATPase was cloned from mouse cerebellum and sequenced. The deduced polypeptide (155 amino acid residues; molecular weight, 15,808) was highly hydrophobic and homologous to the subunits of bovine adrenal medulla, Torpedo marmorata electric lobe, Drosophila and yeast. Glu-139 (supposed to be essential for proton transport) was also conserved as the potential dicyclohexylcarbodiimide binding site. The subunit had four transmembrane segments: Segment II and IV were highly homologous and Glu-139 was located in Segment IV. The roles of the non-conserved regions are discussed. © 1991.

引用

页码：1062 / 1067

页数：6

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