SIMULTANEOUS SWIM-UP SWIM-DOWN OF SPERM IN ASSISTED REPRODUCTION PROCEDURES

被引：5

作者：

ALMAGOR, M

BENBENISHTI, D

ROSENAK, D

MOGLE, Y

YAFFE, H

机构：

[1] IVF and Fertility Laboratory, Department of Obstetrics and Gynecology, Bikur Cholim Hospital, Jerusalem, 91004

来源：

JOURNAL OF ASSISTED REPRODUCTION AND GENETICS | 1993年 / 10卷 / 04期

关键词：

SWIM-UP; SWIM-DOWN; PERCOLL; IN-VITRO FERTILIZATION;

D O I：

10.1007/BF01204939

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Purpose: High-quality motile human spermatozoa were obtained following treatment of semen by simultaneous swim-up into medium and swim-down into an isotonic 40% Percoll solution. Results: This procedure was significantly better than the swim-up method and comparable to discontinuous Percoll gradient centrifugation. Recovery rates of motile sperm were 35% for swim-up, 65% for Percoll gradient centrifugation, and 73% for swim-up/swim-down. Conclusion: The swim-down sperm was inferior to the swim-up sperm in its upward migration capacity but superior in morphology. Spermatozoa obtained by the swim-up/swim-down procedure demonstrated fertilizing ability in IVF, and clinical pregnancies were established. The simultaneous swim-up/swim-down procedure offers an alternative efficient method of simple separation of high-quality motile sperm for various assisted reproduction techniques.

引用

页码：261 / 265

页数：5

共 20 条

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