DIRECT REDUCTION OF NAD(+) BY ELECTROCHEMICAL PROCEDURE AND APPLICATION OF THE REGENERATED NADH TO ENZYME REACTION

被引：11

作者：

YUN, SE ^{[1
]}

TAYA, M ^{[1
]}

TONE, S ^{[1
]}

机构：

[1] OSAKA UNIV,DEPT CHEM ENGN,TOYONAKA,OSAKA 560,JAPAN

来源：

BIOTECHNOLOGY LETTERS | 1994年 / 16卷 / 10期

关键词：

D O I：

10.1007/BF01022402

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Enzymatically active NADH was formed from. NAD(+) directly in the electrochemical system by adopting an anion-charged membrane as a diaphragm and applying the less cathodic potential of -0.7V vs. Ag/AgCl electrode. The direct electrochemical procedure worked favorably as an NADH regenerator in the biochemical reaction system using D-lactate dehydrogenase which transforms pyruvate to D-lactate.

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页码：1053 / 1058

页数：6

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