DOES THE SULFHYDRYL OR THE ADENINE MOIETY OF COA ENHANCE FIREFLY LUCIFERASE ACTIVITY

被引：27

作者：

FORD, SR ^{[1
]}

BUCK, LM ^{[1
]}

LEACH, FR ^{[1
]}

机构：

[1] OKLAHOMA STATE UNIV,NOBLE RES CTR 246B,DEPT BIOCHEM & MOLEC BIOL,STILLWATER,OK 74078

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY | 1995年 / 1252卷 / 02期

关键词：

LUCIFERASE; BIOLUMINESCENCE; COENZYME A; ADENINE NUCLEOTIDE; (FIREFLY);

D O I：

10.1016/0167-4838(95)00150-S

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Light production by firefly luciferase is limited by product release resulting in flash kinetics. Several compounds (CoA, PPi, and nucleotides) transform the flash-form of light production into continuous light production The sulfhydryl group of CoA is required; however, since nucleotides are also active, at least two mechanisms (sites) must exist.

引用

页码：180 / 184

页数：5

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