SEPARATION OF BASIC-PROTEINS BY CAPILLARY ELECTROPHORESIS USING CROSS-LINKED POLYACRYLAMIDE-COATED CAPILLARIES AND CATIONIC BUFFER ADDITIVES

被引：43

作者：

CIFUENTES, A ^{[1
]}

DEFRUTOS, M ^{[1
]}

SANTOS, JM ^{[1
]}

DIEZMASA, JC ^{[1
]}

机构：

[1] CSIC,INST ORGAN CHEM,E-28006 MADRID,SPAIN

来源：

JOURNAL OF CHROMATOGRAPHY A | 1993年 / 655卷 / 01期

关键词：

D O I：

10.1016/0021-9673(93)87011-A

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method for the preparation of fused-silica capillaries with a cross-linked polyacrylamide coating bonded to the internal wall is described. When these capillaries are used with acidic separation buffers containing 0.25 M morpholine as cationic additive to mask the residual effect of the negative charges on the silica surface, efficiencies between 3 . 10(5) and 5 . 10(5) plates/m can be obtained for basic proteins (pI 7-11). The reproducibility of migration times for these capillaries is better than 1% run-to-run and 2% capillary-to-capillary. The capillaries can be used for more than 90 h over the pH range 2-10 without any substantial alteration of the migration time or efficiency for the basic proteins assayed. It is also shown that the capillaries can be stored for more than 90 days in air with no appreciable variation in the migration time or the efficiency for several basic proteins.

引用

页码：63 / 72

页数：10

共 32 条

[1]

ALTRIA KD, 1986, ANAL P, V23, P453

[2] CAPILLARY ZONE ELECTROPHORETIC SEPARATIONS OF PROTEINS IN POLYETHYLENE GLYCOL-MODIFIED CAPILLARIES [J].