PROPIDIUM IODIDE IN-VIVO - AN EARLY MARKER OF NEURONAL DAMAGE IN RAT HIPPOCAMPUS

被引：21

作者：

WILDE, GJC

SUNDSTROM, LE

IANNOTTI, F

机构：

[1] SOUTHAMPTON GEN HOSP,DEPT CLIN NEUROL SCI,SOUTHAMPTON SO16 6YD,HANTS,ENGLAND

[2] UNIV SOUTHAMPTON,DEPT PHYSIOL & PHARMACOL,SOUTHAMPTON SO9 3TU,HANTS,ENGLAND

来源：

NEUROSCIENCE LETTERS | 1994年 / 180卷 / 02期

关键词：

PROPIDIUM IODIDE; KAINIC ACID; HIPPOCAMPUS; NEURONAL DAMAGE; RAT;

D O I：

10.1016/0304-3940(94)90525-8

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

We have investigated the use of the fluorescent exclusion dye propidium iodide as a marker for acutely degenerating cells in vivo, and report here that combined injection of kainic acid and propidium iodide into the lateral cerebral ventricle results in labelling of CA3 pyramidal cells 1 and 6 h after injection. Alternate sections stained with thionin at these early times revealed little evidence of histologically detectable cell damage.

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页码：223 / 226

页数：4

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