DETERMINATION OF STEROID MONO- AND DISULFATES IN HUMAN PLASMA

被引：144

作者：

JANNE, O

VIHKO, R

SJOVALL, J

SJOVALL, K

机构：

[1] Department of Medical Chemistry, University of Helsinki

[2] Department of Chemistry, Karolinska Institutet, Stockholm

来源：

CLINICA CHIMICA ACTA | 1969年 / 23卷 / 03期

关键词：

D O I：

10.1016/0009-8981(69)90340-4

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

A method for the determination of androsterone, dehydroepiandrosterone, epiandrosterone, 5α-androstane-3α,17β-diol, androst-5-ene-3β17α-diol, androst-5-ene-3β,17β-diol, pregnenolone, pregn-5-ene-3β,20α-diol and pregn-5-ene-3β,17α,20α-triol monosulfates and androst-5-ene-3β,17α-diol, androst-5-ene-3β,17β-diol and pregn-5-ene-3β,20α-diol disulfates in human plasma is described. A plasma extract is chromatographed on Sephadex LH-20 and a monosulfate and a disulfate fraction are obtained. After solvolysis, the steroids are purified on a silicic acid column and their trimethylsilyl ethers are analyzed by gas chromatography on QF-1 and SE-30 columns. The specificity of the method was established by mass spectrometric analysis of the effluent from the GLC columns. Concentrations of steroid mono- and disulfates in peripheral plasma of a group of young healthy female and male subjects are given. © 1968.

引用

页码：405 / +

页数：1

共 23 条

[1]

ADLERCREUTZ H, 1968, ANN MED EXP BIOL FEN, V46, P165

[2]

BURSTEIN S, 1958, J BIOL CHEM, V233, P331

[3] KINETICS AND MECHANISM OF SOLVOLYSIS OF STEROID HYDROGEN SULFATES [J].