A series of experiments was performed to elucidate the relationship between cells that form granulocytic colonies in fibrin clot diffusion chambers implanted into the peritoneum (i.p.) of irradiated mice (CFU‐d) and day 7 and day 14 CFU‐c which give rise to colonies after 7 and 14 days in agar cultures in vitro, respectively. Normal human bone marrow cells were cultured in suspension in vitro or in diffusion chambers implanted into irradiated or non‐irradiated mice. During these culture conditions there was an initial decrease in the number of CFU‐c per culture. This was followed by an increase between day 2 and day 7 of culture. No similar increase of neutrophilic CFU‐d was observed. When CFU‐d, day 14 and day 7 CFU‐c in normal marrow were separated by velocity sedimentation and cultured in suspension culture or in diffusion chambers for 7 days, the maximum increase of day 7 and day 14 CFU‐c was observed in slowly sedimenting cell fractions which contained the majority of CFU‐d. After 3 days in suspension culture, the maximum increase of day 14 CFU‐c was found in fractions which also gave rise to maximum numbers of CFU‐c after 7 days. However, day 7 CFU‐c were found in fractions which initially contained the majority of day 14 CFU‐c. No increase in CFU‐d was found in fractions initially containing peak numbers of CFU‐c. Between 53 and 71% of CFU‐c harvested from diffusion chambers in irradiated mice or from suspension cultures were sensitive to pulse incubation with tritiated thymidine, suggesting that the cells were proliferating during these culture conditions. In diffusion chambers implanted into non‐irradiated mice, however, CFU‐c were found to be relatively resistant to this treatment (3–11% sensitive to tritiated thymidine). Thus marked increases in CFU‐c were also observed during experimental conditions, where no significant DNA synthesis was detected. A reproducible time sequence of increase in CFU‐c populations in culture was observed. Day 14 CFU‐c and cells that gave rise to clusters on day 7 in agar increased between day 2 and day 4, whereas day 7 CFU‐c increased between day 4 and day 7. The results suggested that CFU‐d gave rise to CFU‐c in culture and that day 14 CFU‐c were precursors of day 7 CFU‐c. Copyright © 1979, Wiley Blackwell. All rights reserved
