CLONING AND SEQUENCING OF LOL PI, THE MAJOR ALLERGENIC PROTEIN OF RYE-GRASS POLLEN

被引：129

作者：

GRIFFITH, IJ

SMITH, PM

POLLOCK, J

THEERAKULPISUT, P

AVJIOGLU, A

DAVIES, S

HOUGH, T

SINGH, MB

SIMPSON, RJ

WARD, LD

KNOX, RB

机构：

[1] UNIV MELBOURNE,SCH BOT,PARKVILLE,VIC 3052,AUSTRALIA

[2] IMMULOG PHARMACEUT CORP,CAMBRIDGE,MA 02139

[3] WALTER & ELIZA HALL INST MED RES,LUDWIG INST CANC RES,JOINT PROT STRUCT LAB,PARKVILLE,VIC 3052,AUSTRALIA

来源：

FEBS LETTERS | 1991年 / 279卷 / 02期

基金：

英国医学研究理事会;

关键词：

LOLIUM-PERENNE; ALLERGEN; CDNA CLONING; NUCLEOTIDE SEQUENCE; AMINO ACID SEQUENCE; VARIATE NORMAL DISTRIBUTIONS;

D O I：

10.1016/0014-5793(91)80151-R

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have isolated a full length cDNA clone encoding the major glycoprotein allergen Lol pI. The clone was selected using a combination of immunological screening of a cDNA expression library and PCR amplification of Lol pI-specific transcripts. Lol pI expressed in bacteria as a fusion protein shows recognition by specific IgE antibodies present in sera of grass pollen-allergic subjects. Northern analysis has shown that the Lol pI transcripts are expressed only in pollen of rye-grass. Molecular cloning of Lol pI provides a molecular genetic approach to study the structure-function relationship of allergens.

引用

页码：210 / 215

页数：6

共 36 条

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