SIMULTANEOUS DETERMINATION OF TOTAL CHLOROGENIC ACID AND CAFFEINE IN COFFEE BY HIGH-PERFORMANCE GEL-FILTRATION CHROMATOGRAPHY

被引：22

作者：

TRUGO, LC

DEMARIA, CAB

WERNECK, CC

机构：

[1] Department of Biochemistry, Institute of Chemistry, Universidade Federal do Rio de Janeiro, 21944 Rio de Janeiro, C. T. bloco A, Cidade Universitaria

来源：

FOOD CHEMISTRY | 1991年 / 42卷 / 01期

关键词：

D O I：

10.1016/0308-8146(91)90008-C

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

A method based on high performance gel filtration chromatography, using a TSK 3000-SW column, was developed for the simultaneous determination of total chlorogenic acid and caffeine in coffee samples. The method presented good linearities and recoveries for both compounds. Comparison with results obtained by reversed phase chromatography for caffeine and by the AOAC procedure and an adapted reversed phase chromatographic method for chlorogenic acid showed good correlations between the proposed method and the reversed phase procedures (0.997 for caffeine and 0.998 for chlorogenic acid). The AOAC results for chlorogenic acid showed systematically higher values than the proposed method. The proposed method gave a CV(%) of 6.5 for caffeine and 5.0 for chlorogenic acid and is adequate when applied to the analysis of different samples of green, roasted decaffeinated and instant coffees.

引用

页码：81 / 87

页数：7

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