HETERONUCLEAR NMR OF DNA WITH THE HETERONUCLEUS IN NATURAL ABUNDANCE - FACILITATED ASSIGNMENT AND EXTRACTION OF COUPLING-CONSTANTS

被引：56

作者：

SCHMIEDER, P

IPPEL, JH

VANDENELST, H

VANDERMAREL, GA

VANBOOM, JH

ALTONA, C

KESSLER, H

机构：

[1] TECH UNIV MUNICH, INST ORGAN CHEM, LICHTENBERGSTR 4, W-8046 GARCHING, GERMANY

[2] LEIDEN UNIV, GORLAEUS LABS, DEPT ORGAN CHEM, 2300 RA LEIDEN, NETHERLANDS

来源：

NUCLEIC ACIDS RESEARCH | 1992年 / 20卷 / 18期

关键词：

D O I：

10.1093/nar/20.18.4747

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two heteronuclear proton-carbon NMR experiments are applied to the DNA-octamer d(TTGGCCAA)2 with carbon in natural abundance. They lead to a complete assignment of the carbon resonances of the sugars and bases. In addition, several heteronuclear coupling constants, proton-carbon as well as proton-phosphorous and phosphorous-carbon, were determined. The information can be obtained in a resonable measuring time and offers valuable information for a detailed picture of DNA structure.

引用

页码：4747 / 4751

页数：5

共 50 条

[1]

ALTONA C, 1982, RECL TRAV CHIM PAY B, V101, P413

[2] HETERONUCLEAR 2-DIMENSIONAL N-15-NMR AND C-13-NMR STUDIES OF DNA OLIGOMERS AND THEIR NETROPSIN COMPLEXES USING INDIRECT PROTON DETECTION [J].