CALCIUM ALGINATE ENCAPSULATION OF SMALL SPECIMENS FOR TRANSMISSION ELECTRON-MICROSCOPY

被引：12

作者：

PAGE, AM

LAGNADO, JR

FORD, TW

PLACE, G

机构：

[1] Royal Holloway, University of London, Egham, Surrey

[2] Bayer plc, Stoke Poges, SL2 44Y, Stoke Court Slough

来源：

JOURNAL OF MICROSCOPY-OXFORD | 1994年 / 175卷

关键词：

ALGINATE; ENCAPSULATION; CELL CULTURE; IMMUNOCYTOCHEMISTRY; TEM;

D O I：

10.1111/j.1365-2818.1994.tb03480.x

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

A technique of encapsulating small objects in calcium alginate for further processing for transmission electron microscopy is described. Five methods are outlined which enable a variety of specimens including single cells (in suspension and on agar plates), small organisms and monolayers of tissue culture cells to be processed. A method for immunolabelling alginate-entrapped material is also outlined.

引用

页码：166 / 170

页数：5

共 22 条

[1]

[Anonymous], 1986, BASIC TECHNIQUES TRA

[2]

[Anonymous], [No title captured]

[3]

[Anonymous], 1975, GREEN ALGAE STRUCTUR

[4] PLANT-PROTOPLASTS IMMOBILIZED IN CALCIUM ALGINATE - A SIMPLE METHOD OF PREPARING FRAGILE CELLS FOR TRANSMISSION ELECTRON-MICROSCOPY [J].