EXPRESSION OF LOW-MOLECULAR-WEIGHT HSP-70 RELATED POLYPEPTIDES FROM THE SYMBIOTIC SEA-ANEMONE ANEMONIA-VIRIDIS FORSKALL IN RESPONSE TO HEAT-SHOCK

被引：63

作者：

SHARP, VA ^{[1
]}

MILLER, D ^{[1
]}

BYTHELL, JC ^{[1
]}

BROWN, BE ^{[1
]}

机构：

[1] UNIV LEEDS,SCH MED,DEPT CLIN MED,ACAD UNIT PATHOL,LEEDS LS2 9JT,W YORKSHIRE,ENGLAND

来源：

JOURNAL OF EXPERIMENTAL MARINE BIOLOGY AND ECOLOGY | 1994年 / 179卷 / 02期

关键词：

HEAT SHOCK; ANEMONIA-VIRIDIS; HSP-70;

D O I：

10.1016/0022-0981(94)90113-9

中图分类号：

Q14 [生态学（生物生态学）];

学科分类号：

071012 ; 0713 ;

摘要：

Monoclonal antibodies raised against the highly conserved heat shock protein (hsp) 70 failed to detect an equivalent molecular mass homologue in the temperate sea anemone Anemonia viridis and its endosymbiotic zooxanthellae before or after heat shock. Cross-reaction does occur with a heat shock inducible 70 kDa protein in Goniopora djiboutiensis (Vaughan), a subtidal tropical coral. Hsp 70-specific antibodies did recognise 28 kDa and 29 kDa soluble proteins from intertidal A. viridis before and after heat shock. Differential expression of these low molecular mass hsp 70 homologues in intertidal and subtidal populations correlates with thermotolerance. Anemones maintained artificially without endosymbiotic zooxanthellae are capable of synthesising the low molecular mass hsp homologues following heat shock, but do not express them constitutively.

引用

页码：179 / 193

页数：15

共 41 条

[1] THE HEAT-SHOCK RESPONSE IN HYDRA - IMMUNOLOGICAL RELATIONSHIP OF HSP60, THE MAJOR HEAT-SHOCK PROTEIN OF HYDRA-VULGARIS, TO THE UBIQUITOUS HSP70 FAMILY [J].

BOSCH, TCG ;

PRAETZEL, G .

HYDROBIOLOGIA, 1991, 216 :513-517

[2] THERMOTOLERANCE AND SYNTHESIS OF HEAT-SHOCK PROTEINS - THESE RESPONSES ARE PRESENT IN HYDRA-ATTENUATA BUT ABSENT IN HYDRA-OLIGACTIS [J].

BOSCH, TCG ;

KRYLOW, SM ;

BODE, HR ;

STEELE, RE .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (21) :7927-7931

[3]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[4] DAMAGE AND RECOVERY OF CORAL REEFS AFFECTED BY EL-NINO RELATED SEAWATER WARMING IN THE THOUSAND ISLANDS, INDONESIA [J].

BROWN, BE ;

SUHARSONO .

CORAL REEFS, 1990, 8 (04) :163-170

[5] UNCOATING ATPASE IS A MEMBER OF THE 70 KILODALTON FAMILY OF STRESS PROTEINS [J].

CHAPPELL, TG ;

WELCH, WJ ;

SCHLOSSMAN, DM ;

PALTER, KB ;

SCHLESINGER, MJ ;

ROTHMAN, JE .

CELL, 1986, 45 (01) :3-13

[6] 70K HEAT-SHOCK RELATED PROTEINS STIMULATE PROTEIN TRANSLOCATION INTO MICROSOMES [J].

CHIRICO, WJ ;

WATERS, MG ;

BLOBEL, G .

NATURE, 1988, 332 (6167) :805-810

[7]

COLES SL, 1976, PAC SCI, V2, P159

[8]

Cossins AH, 1987, TEMPERATURE BIOL ANI

[9] THE HEAT-SHOCK RESPONSE [J].

CRAIG, EA .

CRC CRITICAL REVIEWS IN BIOCHEMISTRY, 1985, 18 (03) :239-280

[10]

CRAIG EA, 1988, ANNU REV GENET, V22, P631

← 1 2 3 4 5 →