IN-VITRO MEGAKARYOCYTOPOIETIC AND THROMBOPOIETIC ACTIVITY OF C-MPL LIGAND (TPO) ON PURIFIED MURINE HEMATOPOIETIC STEM-CELLS

被引：239

作者：

ZEIGLER, FC

DESAUVAGE, F

WIDMER, HR

KELLER, GA

DONAHUE, C

SCHREIBER, RD

MALLOY, B

HASS, P

EATON, D

MATTHEWS, W

机构：

[1] GENENTECH INC,DEPT BIOL MOLEC,S SAN FRANCISCO,CA 94080

[2] GENENTECH INC,DEPT IMMUNOL,S SAN FRANCISCO,CA 94080

[3] GENENTECH INC,DEPT CARDIOVASC RES,S SAN FRANCISCO,CA 94080

[4] GENENTECH INC,DEPT PHARMACOL,S SAN FRANCISCO,CA 94080

[5] GENENTECH INC,RAF,S SAN FRANCISCO,CA 94080

[6] WASHINGTON UNIV,SCH MED,DEPT PATHOL,ST LOUIS,MO 63110

来源：

BLOOD | 1994年 / 84卷 / 12期

关键词：

D O I：

10.1182/blood.V84.12.4045.bloodjournal84124045

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Recently, the ligand for c-mpl has been identified and cloned, Initial studies of this molecule indicate that it is the platelet regulatory factor, thrombopoietin (TPO). Previous work has indicated that c-mpl is expressed in very immature hematopoietic precursors and thus raised the possibility that TPO may act directly on the hematopoietic stem cell. Therefore, in these studies, we investigate the effects of TPO on hematopoietic stem cell populations isolated from the murine fetal liver and bone marrow. Cocultivation of stem cells with fetal liver stroma gives rise to multilineage expansion of the stem cells but with little or no megakaryocytopoiesis.

引用

页码：4045 / 4052

页数：8

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