REPRESSION OF PREPROTACHYKININ-A PROMOTER ACTIVITY IS MEDIATED BY A PROXIMAL PROMOTER ELEMENT

被引：32

作者：

MENDELSON, SC ^{[1
]}

MORRISON, CF ^{[1
]}

MCALLISTER, J ^{[1
]}

PATERSON, JM ^{[1
]}

DOBSON, SP ^{[1
]}

MULDERRY, PK ^{[1
]}

QUINN, JP ^{[1
]}

机构：

[1] ROYAL EDINBURGH & ASSOCIATED HOSP, MRC, BRAIN METAB UNIT, EDINBURGH EH10 5HF, MIDLOTHIAN, SCOTLAND

来源：

NEUROSCIENCE | 1995年 / 65卷 / 03期

关键词：

D O I：

10.1016/0306-4522(94)00554-I

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The rat preprotachykinin-A promoter, which is able to direct reporter gene expression in adult dorsal root ganglia neurons grown in culture, has no detectable activity in HeLa and PC12 cells. DNAase 1 footprinting and electrophoretic mobility shift analyses with HeLa nuclear extract indicated the presence of a protein complex binding to a region of the rat preprotachykinn-A gene promoter between the TATA box and the major transcriptional start site. We demonstrate that the sequence of the preprotachykinin-A promoter spanning nucleotides -47 to +92 functions to repress reporter gene expression in HeLa and PC12 cells but not in adult rat dorsal root ganglia grown in culture, and that this repression is correlated with a protein(s) binding to the element between the TATA box and major transcription initiation site. These results indicate that the tissue-specific expression of the preprotachykinin-A gene could require the interaction of both positive and negative regulatory DNA elements.

引用

页码：837 / 847

页数：11

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