DETERMINATION OF HUMAN-FIBROBLASTS METABOLISM INVITRO BY GAS-CHROMATOGRAPHY MASS-SPECTROMETRY OF CELL-EXCRETED METABOLITES

被引：5

作者：

ANTOSHECHKIN, AG ^{[1
]}

TATUR, VY ^{[1
]}

PEREVEZENTSEVA, OM ^{[1
]}

MAXIMOVA, LA ^{[1
]}

机构：

[1] ACAD SCI USSR, INST MOLEC GENET, KURCHATOV SQ, MOSCOW 123182, USSR

来源：

ANALYTICAL BIOCHEMISTRY | 1988年 / 169卷 / 01期

关键词：

D O I：

10.1016/0003-2697(88)90253-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In order to develop an approach to the study of cell metabolism in vitro, we undertook the determination of metabolites excreted by human skin diploid fibroblasts into culture medium using high-resolution gas chromatography in combination with mass spectrometry. Chromatographic and mass spectrometric characteristics of 29 metabolites have been obtained, and 11 of the metabolites have been identified. The excreted metabolites reflect the activity of certain metabolic processes in fibroblasts in vitro. A comparison of chromatographic and mass spectrometric characteristics of the cell metabolites and of those excreted from the body in urine showed most of the metabolites excreted by fibroblasts to be different from the urine metabolites. The possibility of secondary conversion of cell metabolites in the organism and the specificity of metabolism in cells of different tissues are discussed.

引用

页码：33 / 40

页数：8

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