INVITRO PRODUCTION OF MONOCLONAL-ANTIBODIES UNDER SERUM-FREE CONDITIONS USING A COMPACT AND INEXPENSIVE HOLLOW FIBER CELL-CULTURE UNIT

被引：15

作者：

KLERX, JPAM

VERPLANKE, CJ

BLONK, CG

TWAALFHOVEN, LC

机构：

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1988年 / 111卷 / 02期

关键词：

D O I：

10.1016/0022-1759(88)90125-1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

引用

页码：179 / 188

页数：10

共 14 条

[1] CONTINUOUS HYBRIDOMA GROWTH AND MONOCLONAL-ANTIBODY PRODUCTION IN HOLLOW FIBER REACTORS SEPARATORS [J].

ALTSHULER, GL ;

DZIEWULSKI, DM ;

SOWEK, JA ;

BELFORT, G .

BIOTECHNOLOGY AND BIOENGINEERING, 1986, 28 (05) :646-658

[2] METHODS FOR GROWTH OF CULTURED-CELLS IN SERUM-FREE MEDIUM [J].

BARNES, D ;

SATO, G .

ANALYTICAL BIOCHEMISTRY, 1980, 102 (02) :255-270

[3] PRODUCTION OF MONOCLONAL-ANTIBODIES IN SERUM FREE MEDIUM [J].

CHANG, TH ;

STEPLEWSKI, Z ;

KOPROWSKI, H .

JOURNAL OF IMMUNOLOGICAL METHODS, 1980, 39 :369-375

[4]

CLEVELAND WL, 1983, J IMMUNOL METHODS, V56, P221

[5] AUTOMATED PRODUCTION OF MONOCLONAL-ANTIBODIES IN A CYTOSTAT [J].

DESTGROTH, SF .

JOURNAL OF IMMUNOLOGICAL METHODS, 1983, 57 (1-3) :121-136

[6] HOLLOW FIBER CELL-CULTURE SYSTEMS FOR ECONOMICAL CELL-PRODUCT MANUFACTURING [J].

HOPKINSON, J .

BIO-TECHNOLOGY, 1985, 3 (03) :225-&

[7]

Jarvis Jr. A.P., 1983, BIOTECHNIQUES, V1, P22

[8] CONTINUOUS CULTURES OF FUSED CELLS SECRETING ANTIBODY OF PREDEFINED SPECIFICITY [J].

KOHLER, G ;

MILSTEIN, C .

NATURE, 1975, 256 (5517) :495-497

[9]

KOVAR J, 1986, METHOD ENZYMOL, V121, P277

[10] SERUM-FREE MEDIUM FOR HYBRIDOMA AND PARENTAL MYELOMA CELL CULTIVATION - A NOVEL COMPOSITION OF GROWTH-SUPPORTING SUBSTANCES [J].

KOVAR, J ;

FRANEK, F .

IMMUNOLOGY LETTERS, 1984, 7 (06) :339-345

← 1 2 →