CALCIFICATION OF DIFFERENTIATING SKELETAL MESENCHYME INVITRO

被引：52

作者：

BINDERMAN, I

GREENE, RM

PENNYPACKER, JP

机构：

[1] Laboratory of Developmental Biology and Anomalies, National Institute of Dental Research, National Institutes of Health, Bethesda

来源：

SCIENCE | 1979年 / 206卷 / 4415期

关键词：

D O I：

10.1126/science.482937

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 [理学]; 0710 [生物学]; 09 [农学];

摘要：

Embryonic limb-bud mesenchyme was induced to calcify in culture by the addition of 3 mM inorganic phosphate to the medium. Phosphate enhanced calcification of the matrix produced by mesenchymal or fibroblast-like cells, whereas no calcification was evident in areas where cartilage had developed. However, calcification was induced throughout the cell layer by altering the cartilage matrix properties with certain enzymes or by changing the phenotypic expression of the cells with vitamin A.

引用

页码：222 / 225

页数：4

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