POLYPEPTIDE COMPONENTS OF THE APAMIN RECEPTOR ASSOCIATED WITH A CALCIUM ACTIVATED POTASSIUM CHANNEL

被引：11

作者：

LEVEQUE, C

MARQUEZE, B

COURAUD, F

SEAGAR, M

机构：

[1] Laboratoire de Biochimie, CNRS UA1179, Faculté de Médicine Nord, 13326 Marseille Cedex 15, Bd. Pierre Dramurd

来源：

FEBS LETTERS | 1990年 / 275卷 / 1-2期

关键词：

Apamin receptor polypeptide; Calcium activation; Potassium channel; Rat brain membrane;

D O I：

10.1016/0014-5793(90)81468-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Photoaflinity labeling of rat brain membraines with [125l]ANPAA-apamin incorporated radioactivity into polypeptides of 86 and 59 kDa and occasionally a more weakly labeled component of 45 kDa. These polypeptides were immunoprecipitated with anti-apamin antibodies and treated with glycosidases. Neither the 86 nor the 59 kDa polypeptide appeared to b %glycoxylated. Partial protocolytic mapping of affinity labeled polypeptides with chymotrypsin or V8 protease generated an identical pattern. Thes results suggest that the 59 and 45 kDa components are not additional subunits of an oligomeric protein but results from cleavage of the 86 kDa polypeptide. © 1990.

引用

页码：185 / 189

页数：5

共 18 条

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