FORMATION OF DIHYDRODIOLS FROM BENZO[A]PYRENE BY OXIDATION WITH AN ASCORBIC ACID-FERROUS SULFATE-EDTA SYSTEM

被引：15

作者：

HEWER, A

RIBEIRO, O

WALSH, C

GROVER, PL

SIMS, P

机构：

[1] The Chester Beatty Research Institute, Institute of Cancer Research: Royal Cancer Hospital, London, SW3 6JB, Fulham Road

来源：

CHEMICO-BIOLOGICAL INTERACTIONS | 1979年 / 26卷 / 02期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1016/0009-2797(79)90018-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In the oxidation of benzo [a] pyrene in an ascorbic acid-ferrous sulphate-EDTA system, our dihydrodiola were detected. Three, trans-4,5-dihydro-4,5-dihydroxybenzo [a] pyrene, trans-7,8-dihydro-7,8-dihydroxybenzo[a] pyrene and trans-9,10-dihydro-9,10-dihydroxybenzo[a] pyrene were identified by their UV spectra and by direct comparisons of their chromatographic properties, using HPLC, with those of the authentic compounds. The fourth compound appeared to be trans-11, 12-dihydro-11,12-dihydroxybenzo[a] pyrene since its ultraviolet spectrum was identical to that of the cis-dihydrodiol. Time-course experiments showed that the maximum amounts of products were obtained after 8 h of oxidation. A re-examination of the dihydrodiols formed from benzo[a]pyrene by rat-liver microsomal fractions failed to show the formation of the trans-11,12-dihydrodiol. © 1979.

引用

页码：147 / 154

页数：8

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