FOURIER-TRANSFORM HOLOGRAPHIC MICROSCOPE

被引：118

作者：

HADDAD, WS ^{[1
]}

CULLEN, D ^{[1
]}

SOLEM, JC ^{[1
]}

LONGWORTH, JW ^{[1
]}

MCPHERSON, A ^{[1
]}

BOYER, K ^{[1
]}

RHODES, CK ^{[1
]}

机构：

[1] LOS ALAMOS NATL LAB, DIV THEORET, LOS ALAMOS, NM 87545 USA

来源：

APPLIED OPTICS | 1992年 / 31卷 / 24期

关键词：

MICROHOLOGRAPHY; FOURIER-TRANSFORM MICROHOLOGRAPHY; BIOLOGICAL SAMPLES; GLYCEROL MICRODROP; RESOLUTION; NUMERICAL RECONSTRUCTION; REFERENCE SCATTERER;

D O I：

10.1364/AO.31.004973

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We describe a holographic microscope with a spatial resolution approaching the diffraction limit. The instrument uses a tiny drop of glycerol as a lens to create the spherically diverging reference illumination necessary for Fourier-transform holography. Measurement of the point-spread function, which is obtained by imaging a knife edge in dark-field illumination, indicates a transverse resolution of 1.4-mu-m with wavelength lambda = 514.5 nm. Longitudinal resolution is obtained from the holograms by the numerical equivalent of optical sectioning. We describe the method of reconstruction and demonstrate the microscope's capability with selected biological specimens. The instrument offers two unique capabilities: (1) it can collect three-dimensional information in a single pulse of light, avoiding specimen damage and bleaching; and (2) it can record three-dimensional motion pictures from a series of light pulses. The conceptual design is applicable to a broad range of wavelengths and we discuss extension to the x-ray regime.

引用

页码：4973 / 4978

页数：6

共 19 条

[1] A NEW MICROSCOPIC PRINCIPLE
GABOR, D
[J]. NATURE, 1948, 161 (4098) : 777 - 778
[2] MICROSCOPY BY RECONSTRUCTED WAVE-FRONTS
GABOR, D
[J]. PROCEEDINGS OF THE ROYAL SOCIETY OF LONDON SERIES A-MATHEMATICAL AND PHYSICAL SCIENCES, 1949, 197 (1051) : 454 - +
[3] GIBSON SF, 1991, J OPT SOC AM A, V8, P1601, DOI 10.1364/JOSAA.8.001601
[4] HADDAD WS, 1988, OSA P SHORT WAVELENG, V2, P284
[5] HADDAD WS, 1988, XRAY MICROSCOPY, V2, P284
[6] HADDAD WS, 1987, ELECTRONICS IMAGING, V2, P683
[7] JENKINS FA, 1976, FUNDAMENTALS OPTICS, P71
[8] LEITH EN, 1965, J OPT SOC AM, V55, P595
[9] LEITH EN, 1965, J OPT SOC AM, V55, P569
[10] MCNULTY I, 1992, XRAY MICROSCOPY, V3, P251

← 1 2 →