DETECTION OF THE OUTER-MEMBRANE LIPOPROTEIN-I AND ITS GENE IN FLUORESCENT AND NONFLUORESCENT PSEUDOMONADS - IMPLICATIONS FOR TAXONOMY AND DIAGNOSIS

被引:29
作者
DEVOS, D
LIM, A
DEVOS, P
SARNIGUET, A
KERSTERS, K
CORNELIS, P
机构
[1] VRIJE UNIV BRUSSELS,ALGEMENE BIOL LAB,PAARDENSTR 65,B-1640 RHODE ST GENESE,BELGIUM
[2] STATE UNIV GHENT,MICROBIOL LAB,B-9000 GHENT,BELGIUM
[3] INRA,CTR RECH RENNES,PATHOL VEGETALE STN,F-35650 LE RHEU,FRANCE
来源
JOURNAL OF GENERAL MICROBIOLOGY | 1993年 / 139卷
关键词
D O I
10.1099/00221287-139-9-2215
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The open reading frame of the OprI lipoprotein gene from Pseudomonas aeruginosa was amplified by polymerase chain reaction (PCR) starting from purified DNA or colony lysates. A fragment of the expected size (249 bp) was detected in all P. aeruginosa strains from various clinical and geographical origins. The gene could only be amplified in pseudomonads of rRNA group I which are considered to be the authentic genus Pseudomonas. Digestions with HaeIII, PvuII and SphI of the amplified fragments demonstrated a sequence variation in the oprI gene. Colony, dot and Western blots with two monoclonal antibodies (mAbs) against the lipoprotein I confirmed our PCR results. These findings open interesting perspectives for the molecular taxonomy of the genus Pseudomonas and the development of diagnostic tools.
引用
收藏
页码:2215 / 2223
页数:9
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