CELL GROWTH AND POPULATION ACTIVITY OF SACCHAROMYCES CEREVISIAE IN 2-STAGE CONTINUOUS CULTIVATION

被引：4

作者：

BERAN, K

ZEMANOVA, J

机构：

[1] Department of Technical Microbiology, Institute of Microbiology, Czechoslovak Academy of Sciences

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 1969年 / 11卷 / 05期

关键词：

D O I：

10.1002/bit.260110511

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Continuous culture in a cascade of vessels with the addition of supplemental nutrients to any stage permits adjustment of the physiological state of the culture in each stage to best achieve a desired performance goal. The yeast Saccharomyces cerevisiae in two‐stage continuous cultivation was selected as a model system. With conditions in the first stage held constant‐ at a selected glucose concentration in the feed stream, dilution rate for the second stage was varied. Cell numbers, dry weight, glucose concentration, respiration coefficient, and titers of several enzymes were determined. The seed rate was defined as the ratio of glucose concentration in the feeds to stage 1 and to stage 2. At low seed rates, the calculated specific growth rate in the second stage was proportional to dilution rate. At higher seed rates, the specific growth rate based on dry weight behaved differently from that based on cell numbers, and the dependence on dilution rate was not linear. Copyright © 1969 John Wiley & Sons, Inc.

引用

页码：853 / &

共 16 条

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