LOSS OF GROWTH INHIBITORY ACTIVITY OF TGF-BETA TOWARD NORMAL HUMAN MAMMARY EPITHELIAL-CELLS GROWN WITHIN COLLAGEN GEL MATRIX

被引:15
作者
TAKAHASHI, K
SUZUKI, K
ONO, T
机构
[1] Department of Biochemistry, Kanagawa Cancer Center Research Institute, Asahi-ku, Yokohama, 241
关键词
D O I
10.1016/S0006-291X(05)80919-2
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
TGF-β at concentrations in the range from 0.1 to 10 ng/ml gave significant growth inhibition of nonmalignant human mammary epithelial cells (HMEC) but not of malignant HMEC grown in monolayer cultures in serum-free medium. However, no growth inhibition of the nonmalignant cells was observed when the cells were cultivated within a type-I collagen gel matrix either adhering to a plastic substratum or floating on the medium. Within floating collagen gels, both nonmalignant and malignant HMEC formed a cell mass having radial extensions, and TGF-β at 1 or 10 ng/ml prevented the formation of extensions only in the nonmalignant HMEC. © 1990 Academic Press, Inc.
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页码:1239 / 1247
页数:9
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