FURTHER EVIDENCE FOR THE EXISTENCE OF LYMPHOCYTE-B SUB-POPULATIONS

被引:3
作者
GILLETTE, RW
机构
[1] Cancer Center of Hawaii, Honolulu, HI 96822
关键词
D O I
10.1016/0008-8749(79)90359-9
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
We have studied the homing properties of B lymphocytes by using 51Cr-labeled lymphoid cells obtained from athymic, nu/nu mice, and animals made T-lymphocyte deficient by thymectomy and lethal irradiation followed by reconstitution with syngeneic bone marrow. Comparison was made to the patterns of distribution observed when cell preparations containing normal numbers of T and B lymphocytes were migrated. A small but significant percentage of labeled lymphocytes from lymph nodes, spleen, Peyer's Patches, and bone marrow of T-cell-deficient animals was shown to be lymph node seeking. Secondary transfers of lymph node cells from primary recipients caused enrichment of this lymph node-seeking population. Treatment of T-lymphocyte-deficient lymphoid cell preparations with neuraminidase reduced the percentages of cells homing to the lymph nodes. The data showed that B lymphocytes exhibit unique homing properties when injected into normal recipients. In addition, direct comparison of the homing patterns of B lymphocytes prepared from spleen and lymph nodes of athymic mice revealed differences suggesting that these lymphoid organs contained unique mixtures of at least two different kinds of B cell. The evidence supports the notion that the B-lymphocyte populations contain at least two subpopulations, one of which possesses the ability to home to lymph nodes. © 1979.
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页码:26 / 35
页数:10
相关论文
共 24 条
[1]  
ANDERSSON LC, 1975, J IMMUNOL, V114, P1226
[2]   DETECTION OF HOMOGRAFT SENSITIVITY IN MICE BY ELIMINATION OF CHROMIUM-51 LABELED LYMPH NODE CELLS [J].
BAINBRID.DR ;
GOWLAND, G .
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1966, 129 (A1) :257-+
[3]   MIGRATORY PATTERNS OF B-LYMPHOCYTES .1. FATE OF CELLS FROM CENTRAL AND PERIPHERAL LYMPHOID ORGANS IN RABBIT AND ITS SELECTIVE ALTERATION BY ANTI-IMMUNOGLOBULIN [J].
DURKIN, HG ;
CAPORALE, L ;
THORBECKE, GJ .
CELLULAR IMMUNOLOGY, 1975, 16 (02) :285-300
[4]   KINETICS OF LYMPHOCYTE RECIRCULATION WITHIN RAT SPLEEN [J].
FORD, WL .
CELL AND TISSUE KINETICS, 1969, 2 (03) :171-&
[5]  
GILLETTE RW, 1971, J RETICULOENDOTH SOC, V10, P223
[6]   UNIQUE HOMING PROPERTIES OF NONADHERENT PERITONEAL CELLS [J].
GILLETTE, RW ;
BOONE, CW ;
SWANSON, MH .
CELLULAR IMMUNOLOGY, 1973, 9 (03) :445-452
[7]   IDENTIFICATION OF MARROW-DERIVED AND THYMUS-DERIVED SMALL LYMPHOCYTES IN LYMPHOID-TISSUE AND THORACIC-DUCT LYMPH OF NORMAL RATS [J].
HOWARD, JC ;
HUNT, SV ;
GOWANS, JL .
JOURNAL OF EXPERIMENTAL MEDICINE, 1972, 135 (02) :200-&
[9]  
Lance E M, 1970, Ciba Found Study Group, V36, P73
[10]   SEGREGATION OF LYMPHOCYTE POPULATIONS THROUGH DIFFERENTIAL MIGRATION [J].
LANCE, EM ;
TAUB, RN .
NATURE, 1969, 221 (5183) :841-&