NEW SPECTROPHOTOMETRIC CUVETTE HOLDER FOR LOW-TEMPERATURE STUDIES - ITS APPLICATION TO THE STUDY OF CARBONMONOXY-HEMOGLOBIN OXIDATION RATE

被引:4
作者
MOSCA, A [1 ]
MUSETTI, A [1 ]
SAMAJA, M [1 ]
机构
[1] IST SCI SAN RAFFAELE,MILAN,ITALY
来源
JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS | 1979年 / 1卷 / 06期
关键词
antifreezing agents; carbonmonoxyhemoglobin oxidation; intermediate compounds; subzero spectrophotometry;
D O I
10.1016/0165-022X(79)90022-8
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A new spectrophotometric cuvette holder to be used to subzero temperature is described. The device is easily adaptable to a commercial spectrophotometer and it was checked down to -40°C. Satisfactory mixing of the reactants contained in the cuvette at low temperatures is attained using a special stirrer and suitable solution volumes. The rate of carbonmonoxyhemoglobin oxidation by K3Fe(CN)6 at different subzero temperatures has been studied using this apparatus; the results are in aggrement with the extrapolated data at room temperature. © 1979.
引用
收藏
页码:319 / 326
页数:8
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