NEW SPECTROPHOTOMETRIC CUVETTE HOLDER FOR LOW-TEMPERATURE STUDIES - ITS APPLICATION TO THE STUDY OF CARBONMONOXY-HEMOGLOBIN OXIDATION RATE

被引：4

作者：

MOSCA, A ^{[1
]}

MUSETTI, A ^{[1
]}

SAMAJA, M ^{[1
]}

机构：

[1] IST SCI SAN RAFFAELE,MILAN,ITALY

来源：

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS | 1979年 / 1卷 / 06期

关键词：

antifreezing agents; carbonmonoxyhemoglobin oxidation; intermediate compounds; subzero spectrophotometry;

D O I：

10.1016/0165-022X(79)90022-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A new spectrophotometric cuvette holder to be used to subzero temperature is described. The device is easily adaptable to a commercial spectrophotometer and it was checked down to -40°C. Satisfactory mixing of the reactants contained in the cuvette at low temperatures is attained using a special stirrer and suitable solution volumes. The rate of carbonmonoxyhemoglobin oxidation by K3Fe(CN)6 at different subzero temperatures has been studied using this apparatus; the results are in aggrement with the extrapolated data at room temperature. © 1979.

引用

页码：319 / 326

页数：8

共 6 条

[1] STUDIES ON OXIDATION-REDUCTION POTENTIALS OF HEME PROTEINS .4. KINETICS OXIDATION OF HEMOGLOBIN AND MYOGLOBIN BY FERRICYANIDE [J].