GROWTH-FACTOR STIMULATION OF PROLIFERATING CELL NUCLEAR ANTIGEN (PCNA) IN HUMAN BREAST EPITHELIUM IN ORGAN-CULTURE

被引：22

作者：

MONAGHAN, P

PERUSINGHE, NP

NICHOLSON, RI

MCCLELLAND, RA

OHARE, MJ

LANE, DP

JAYATILAKE, H

GUSTERSON, BA

机构：

[1] UNIV WALES COLL MED,TENOVUS INST CANC RES,CARDIFF CF4 4XX,S GLAM,WALES

[2] UNIV DUNDEE,INST MED SCI,DEPT BIOCHEM,DUNDEE DP1 4HN,SCOTLAND

来源：

CELL BIOLOGY INTERNATIONAL REPORTS | 1991年 / 15卷 / 07期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1016/0309-1651(91)90003-2

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Normal human breast explants were maintained in serum-free culture for 7 days in the presence of either insulin hydrocortisone and cholera toxin (I/H/CT), epidermal growth factor (EGF) or transforming growth factor-α (TGF-α). Explants were labelled with [3H]thymidine, fixed in methacarn and processed for autoradiography. Parallel sections were immunolabelled with anti-PCNA antibody and analysed with a CAS 200 image analyser. Thymidine labelling index (TLI) and PCNA expression produced similar results with both indices increased in response to I/H/CT, EGF and TGF-α. In sections double labelled for PCNA and autoradiography the majority of labelled cells were positive for both markers. © 1991.

引用

页码：561 / 570

页数：10

共 27 条

[1] PHENOL RED IN TISSUE-CULTURE MEDIA IS A WEAK ESTROGEN - IMPLICATIONS CONCERNING THE STUDY OF ESTROGEN-RESPONSIVE CELLS IN CULTURE [J].