BIOCHEMICAL-PROPERTIES OF RAT PROTEIN-KINASE C-ETA EXPRESSED IN COS CELLS

被引：41

作者：

DEKKER, LV ^{[1
]}

PARKER, PJ ^{[1
]}

MCINTYRE, P ^{[1
]}

机构：

[1] IMPERIAL CANC RES FUND,PROT PHOSPHORYLAT LAB,LONDON WC2A 3PX,ENGLAND

来源：

FEBS LETTERS | 1992年 / 312卷 / 2-3期

关键词：

PROTEIN KINASE-C; PROTEIN KINASE C-ETA; PHOSPHORYLATION;

D O I：

10.1016/0014-5793(92)80934-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Using a PKC-epsilon cDNA probe a cDNA for PKC-eta has been cloned from a rat lung cDNA library. When expressed in COS cells, rat PKC-eta appeared as an 84 kDa protein. PKC-eta expressed in COS cells, was solubilized by 1% Triton X-100 and purified away from the endogenous PKC-alpha by ammonium sulphate fractionation. The activity of this PKC-eta preparation was characterized with respect to cofactor dependence and substrate specificity. Various PKC pseudosubstrate peptides are phosphorylated by PKC-eta in a phospholipid and TPA-dependent but calcium-independent manner. The polypeptide histone IIIS is a poor substrate.

引用

页码：195 / 199

页数：5

共 26 条

[1] ISOLATION AND CHARACTERIZATION OF PKC-L, A NEW MEMBER OF THE PROTEIN-KINASE C-RELATED GENE FAMILY SPECIFICALLY EXPRESSED IN LUNG, SKIN, AND HEART [J].

BACHER, N ;

ZISMAN, Y ;

BERENT, E ;

LIVNEH, E .

MOLECULAR AND CELLULAR BIOLOGY, 1991, 11 (01) :126-133

[2] RAPID PRODUCTION OF FULL-LENGTH CDNAS FROM RARE TRANSCRIPTS - AMPLIFICATION USING A SINGLE GENE-SPECIFIC OLIGONUCLEOTIDE PRIMER [J].

FROHMAN, MA ;

DUSH, MK ;

MARTIN, GR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (23) :8998-9002

[3] THE PROTEIN-KINASE C-RELATED PKC-L(ETA) GENE-PRODUCT IS LOCALIZED IN THE CELL-NUCLEUS [J].

GREIF, H ;

BENCHAIM, J ;

SHIMON, T ;

BECHOR, E ;

ELDAR, H ;

LIVNEH, E .

MOLECULAR AND CELLULAR BIOLOGY, 1992, 12 (03) :1304-1311

[4] THE ROLE OF PROTEIN-KINASE-C IN TRANSMEMBRANE SIGNALING [J].

KIKKAWA, U ;

NISHIZUKA, Y .

ANNUAL REVIEW OF CELL BIOLOGY, 1986, 2 :149-178

[5] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+

[6] PROTEIN-KINASE-C GROUP-B MEMBERS PKC-DELTA, PKC-EPSILON, PKC-ZETA AND PKC-L(ETA) - COMPARISON OF PROPERTIES OF RECOMBINANT PROTEINS INVITRO AND INVIVO [J].

LIYANAGE, M ;

FRITH, D ;

LIVNEH, E ;

STABEL, S .

BIOCHEMICAL JOURNAL, 1992, 283 :781-787

[7] PURIFICATION AND CHARACTERIZATION OF BOVINE BRAIN PROTEIN KINASE-C ISOTYPE-ALPHA, ISOTOPE-BETA AND ISOTOPE-GAMMA [J].

MARAIS, RM ;

PARKER, PJ .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1989, 182 (01) :129-137

[8]

Merill C., 1980, SCIENCE, V211, P1437

[9] THE ROLE OF PROTEIN KINASE-C IN CELL-SURFACE SIGNAL TRANSDUCTION AND TUMOR PROMOTION [J].

NISHIZUKA, Y .

NATURE, 1984, 308 (5961) :693-698

[10] THE MOLECULAR HETEROGENEITY OF PROTEIN KINASE-C AND ITS IMPLICATIONS FOR CELLULAR-REGULATION [J].

NISHIZUKA, Y .

NATURE, 1988, 334 (6184) :661-665

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