A NUCLEAR FACTOR THAT BINDS PURINE-RICH, SINGLE-STRANDED OLIGONUCLEOTIDES DERIVED FROM S1-SENSITIVE ELEMENTS UPSTREAM OF THE CFTR GENE AND THE MUC1 GENE

被引：35

作者：

HOLLINGSWORTH, MA ^{[1
]}

CLOSKEN, C ^{[1
]}

HARRIS, A ^{[1
]}

MCDONALD, CD ^{[1
]}

PAHWA, GS ^{[1
]}

MAHER, LJ ^{[1
]}

机构：

[1] UNIV OXFORD,JOHN RADCLIFFE HOSP,INST MOLEC MED,OXFORD OX3 9DU,ENGLAND

来源：

NUCLEIC ACIDS RESEARCH | 1994年 / 22卷 / 07期

关键词：

D O I：

10.1093/nar/22.7.1138

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have identified two regions of non-random purine/pyrimidine strand asymmetry that were nearly identical in sequence in the 5' flanking (promoter) regions of the human cystic fibrosis transmembrane conductance regulator (CFTR) gene and the human MUC1 gene. These regions contain perfect mirror repeat elements, a sequence motif previously found to be associated with the formation of H-DNA conformations. In this report we demonstrate that a single-stranded non-B DNA conformation exists at low pH in supercoiled plasmids containing the similar mirror repeat elements, and that S1 nuclease digestion maps the single-stranded region to the position of the mirror repeats. In addition, we identify a nuclear protein of approximately 27 kD that binds to single-stranded oligonucleotides corresponding to the purine-rich strand of this region, but not to the pyrimidine-rich strands or to double-stranded oligonucleotides with corresponding purine/pyrimidine strand asymmetry.

引用

页码：1138 / 1146

页数：9

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