PROPERTIES OF GLUTAMATE RECEPTORS ARE MODIFIED DURING LONG-TERM DEPRESSION IN RAT CEREBELLAR PURKINJE-CELLS

被引:28
作者
HEMART, N [1 ]
DANIEL, H [1 ]
JAILLARD, D [1 ]
CREPEL, F [1 ]
机构
[1] UNIV PARIS 11,CNRS,URA 1121,BAT 440,F-91405 ORSAY,FRANCE
关键词
CEREBELLUM; LONG-TERM-DEPRESSION; AMPA RECEPTORS; DESENSITIZATION; ANIRACETAM;
D O I
10.1016/0168-0102(94)90145-7
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Long-term depression (LTD) of synaptic transmission at parallel fiber (PF)-Purkinje cell (PC) synapses occurs when these synapses are activated in conjunction with direct activation of voltage-gated calcium (Ca2+) channels of PCs. In the present study, we have used Aniracetam to test whether the expression of LTD at PF-PC synapses is due to a genuine modification of properties of alpha-amino-3-hydroxy-5-methyl-isoxazole-4-propionate (AMPA) receptors of these neurons. Whole-cell recordings of PF-mediated EPSCs were performed in thin slices taken from 16-22-day-old rats. In all tested cells, bath application of Aniracetam potentiated PF-mediated EPSCs and prolonged their decay without notably changing their rising phase. On the other hand, Aniracetam prevented the induction of LTD by a pairing protocol with Ca2+ spikes and, conversely, the nootropic compound had a larger potentiating effect on PF-mediated EPSCs during expression of LTD than normally, when this change in synaptic efficacy had been induced prior to Aniracetam application. These data strongly suggest that LTD involves a desensitization of postsynaptic AMPA receptors at PF-PC synapses, or, at least, a change in their functional characteristics.
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页码:213 / 221
页数:9
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