OPTIMIZATION OF CCL64-BASED BIOASSAY FOR TGF-BETA

被引：17

作者：

GARRIGUEANTAR, L ^{[1
]}

BARBIEUX, I ^{[1
]}

LIEUBEAU, B ^{[1
]}

BOISTEAU, O ^{[1
]}

GREGOIRE, M ^{[1
]}

机构：

[1] INST BIOL, INSERM, U419, F-44035 NANTES, FRANCE

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1995年 / 186卷 / 02期

关键词：

TRANSFORMING GROWTH FACTOR BETA; BIOASSAY;

D O I：

10.1016/0022-1759(95)00151-Y

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Transforming growth factor beta (TGF-beta) is released by a variety of cells and known to be involved in many different processes including the immune response, wound healing and carcinogenesis. As most experimental investigations have been based on quantitative analysis of TGF-beta production using a bioassay, it seemed important to test the validity and limitations of this method. This paper analyses several parameters that may impair TGF-beta quantification by bioassay. Recommendations are made concerning the influence of technical parameters and the presence of other cytokines (EGF and bFGF) commonly released by cultured cells to which the MvlLu mink lung epithelial cell line (CCL64) is sensitive.

引用

页码：267 / 274

页数：8

共 26 条

[1] A RAPID COLORIMETRIC BIOASSAY FOR TRANSFORMING GROWTH-FACTOR-BETA (TGF-BETA) USING A MICROWELL PLATE READER [J].