INTERGENIC 16S RIBOSOMAL-RNA GENE (RDNA)-23S RDNA SEQUENCE LENGTH POLYMORPHISMS IN MEMBERS OF THE FAMILY LEGIONELLACEAE

被引：21

作者：

HOOKEY, JV

BIRTLES, RJ

SAUNDERS, NA

机构：

[1] CENT PUBL HLTH LAB, MOLEC BIOL LAB, DEPT MICROBIOL REAGENTS, LONDON NW9 5HT, ENGLAND

[2] CENT PUBL HLTH LAB, RESP & SYST INFECT LAB, LEGIONELLA REFERENCE UNIT, LONDON NW9 5HT, ENGLAND

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1995年 / 33卷 / 09期

关键词：

D O I：

10.1128/JCM.33.9.2377-2381.1995

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A method based on PCR amplification of the 16S rRNA gene (rDNA)-23S rDNA intergenic regions was developed for the identification of species within the family Legionellaceae. The sizes of the PCR products varied from 1,353 to 350 bp. Strains of Legionella pneumophila were characterized as having products of approximately 900 and 530 bp, and L.birminghamensis had products of 1,390, 960, and 380 bp, Of the 38 species of legionellae examined, only 7 were indistinguishable (L. erythra from L. rubrilucens, L. anisa or L. cherrii from L. tucsonensis, and L. quateirensis from L. shakespearei). Two environmental isolates were identified as L. pneumophila. Strain LLAP-3, which was a symbiont of amoebae, could not be associated with any Legionella sp, studied.

引用

页码：2377 / 2381

页数：5

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