ON THE ANALYSIS OF HIGH-ORDER MOMENTS OF FLUORESCENCE FLUCTUATIONS

被引：123

作者：

QIAN, H

ELSON, EL

机构：

[1] Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St. Louis, Missouri

来源：

BIOPHYSICAL JOURNAL | 1990年 / 57卷 / 02期

关键词：

D O I：

10.1016/S0006-3495(90)82539-X

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

A simple, straightforward analysis to characterize the distribution of aggregate sizes in a reversible aggregation system at equilibrium is presented. The method, an extension of fluorescence correlation spectroscopy (FCS), is based on measurements of higher order moments of spontaneous fluctuations of fluorescence intensity emitted from a defined open region of the sample. These fluctuations indicate fluctuations of the numbers of the fluorescent molecules in the observation region. Shot noise resulting from the random character of fluorescence emission and from the photoelectric detection system is modeled as a Poisson distribution and is subtracted from the measured photon count fluctuation moments to yield the desired fluorescence fluctuation moments. This analysis can also be used to estimate the fraction of immobile fluorophores in FCS measurements. © 1990, The Biophysical Society. All rights reserved.

引用

页码：375 / 380

页数：6

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